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        产品展示您现在的位置: 首页 > 产品展示 > 仪器 > ABI PCR仪 > StepOnePlusABI/实时荧光定量PCR仪/4376598/现货



        ABI/实时荧光定量PCR仪/4376598/现货The StepOnePlus™ Real-Time PCR System is a 96-well Real-Time PCR instrument perfect for both first-time and experienced users.

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        Calibrated Dye:FAM™, JOE™, NED, ROX™, SYBR® Green I, TAMRA™, VIC®
        Capacity:1 x 96-well plate, 12 x 8-tube strips, 96 x 0.1 ml tubes
        Detection Method:Primer-Probe Detection, SYBR
        Dimensions:24.6 cm/9.7 in.(W) x 42.7 cm/16.8 in.(D) x 51.2 cm/20.2 in.(H)
        Dynamic Range:Linear Dynamic Range greater than 9 log units (detection)
        External Computer:Notebook
        For Use With (Equipment):StepOnePlus™
        Format:0.1 ml tubes, 8-tube strips, 96-well plate
        Green Features:Sustainable packaging
        High Throughput Compatibility:Multiplexing
        Optics:4 emission filters, Blue LED excitation source, photodiode
        Passive Reference Dye:No ROX, ROX (Pre-mixed), ROX (Separate Tube)
        Peak Block Ramp Rate:4.6°C⁄sec
        Product Line:StepOnePlus™
        Product Size:1 system
        Reaction Speed:Fast, Standard
        Reaction Volume Range:10-30 µl (Standard curve experiments: 40 µl in standard mode is validated)
        Run Time:<2 hrs⁄run (Standard Mode), <40 min⁄run (Fast Mode)
        Sample Ramp Rate:Fast mode: ± 2.2°C⁄sec, Standard mode: ± 1.6°C⁄sec
        Sensitivity:1 copy
        Temperature Accuracy:0.25°C (35 to 95°C) of display temperature
        Temperature Range (Metric):4-100°C
        Temperature Uniformity:0.25°C (35 to 95°C) of setpoint/display temperature
        Thermal Cycling System:Peltier-Based System
        Weight:24 kg (53 lbs.)



        The StepOnePlus™ Real-Time PCR System is a 96-well Real-Time PCR instrument perfect for both first-time and experienced users. The StepOnePlus™ Real-Time PCR System can be setup in a variety of configurations and comes ready to use, out of the box, with intuitive data analysis and instrument control software. Utilizing robust LED based 4-color optical recording, the StepOnePlus™ Real-Time PCR System is designed to deliver precise, quantitative Real-Time PCR results for a variety of genomic research applications. We supply a Dell™ Laptop with the StepOnePlus™ Real-Time PCR System.

        Features of the StepOnePlus™ Real-Time PCR System include:

        • Advanced software and instrumentation for performing a wide array of genomic assays
        • Sensitive 4-color optical LED recording system
        • Intuitive and robust software perfect for both first-time and advanced users
        • Simple and flexible instrument set-up and usage

        New StepOne™ Software
        The StepOne™ Software included with the StepOnePlus™ Real-Time PCR System runs on the Windows® XP operating system and provides instrument control, data collection, and data analysis capabilities (analysis software is also compatible with Windows® 7). This latest version includes capabilities to collect melt curve data for High Resolution Melt (HRM) applications and the option to export in Real-Time PCR Data Markup Language (RDML) for compatibility with MIQE guidelines.

        Rapid and Simple Assay Set-Up
        This remarkably simple Real-Time PCR system is designed with an LCD touchscreen and a user-friendly interface that brings the power of genetic studies to researchers new to Real-Time PCR (Fig 1). The intuitive software and protocol wizards help guide new users through their Real-Time PCR experiments. The StepOnePlus™ Real-Time PCR System also includes a quick-start setup so that you start a run immediay and enter plate information at a later time.

        Sensitive LED-Based 4 Color Fluorescence Reading
        The StepOnePlus™ Real-Time PCR System utilizes a long-life LED-based optical system that can record fluorescence from FAM™/SYBR® Green, VIC®/JOE™, NED™/TAMRA™, and ROX™ dyes. This cost-effective, 4-color, 96-well system delivers precise, quantitative Real-Time PCR results and saves data from all filters in every run without depending on a computer or plate setup. It can discriminate between 2 populations of 5,000 and 10,000 template copies of a TaqMan® assay with 99.7% confidence.

        Compatible with Many Genomic Analysis Techniques
        Perform a variety of standard and demanding genetic analysis research techniques on one instrument using the user friendly StepOnePlus™ Real-Time PCR System and StepOne™ Software.

        The StepOnePlus™ System supports many Real-Time PCR applications, including the following:

        • SNP Genotyping
        • Gene Expression Analysis
        • MicroRNA Expression
        • Protein Expression
        • Translocation Analysis
        • Gene Detection
        • Viral Load Analysis

        The included StepOne™ Software supports a variety of analysis methods, including the following:

        • Standard Curve (absolute quantitation)
        • Relative Standard Curve
        • Comparative Ct (ΔΔCt) (relative quantitation)
        • Genotyping and Presence/Absence
        • Melt Curve Analysis (as a standalone application)
        • High Resolution Melting (as a standalone application)

        Versatile Instrument Configuration Options
        The ultra-compact footprint of a StepOnePlus™ System can be installed in multiple distinct configurations, providing unmatched flexibility and convenience that can allow a fit in any laboratory (Fig 2). The StepOnePlus™ Real-Time PCR System can be used via a PC, with a PC connected to a Local Area Network (LAN), or as a stand-alone instrument (PC-free). Each StepOnePlus™ Real-Time PCR System is factory-calibrated for optical and thermal accuracy, so simply remarkable Real-Time PCR results can be obtained right out of the box.



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